Staining:Article Title: Hybrid and SARS-CoV-2-vaccine immunity in kidney transplant recipients.
Article Snippet: .. Detection of SARS-CoV-2 specific memory B cells Spike-specific B cells were detected using either sequential staining of biotinylated Recombinant WT SARS-CoV-2 Spike-Trimer and RBD (HEK) (Miltenyi) conjugated with streptavidin-PE or streptavidin-BV786 respectively were combined with probes already conjugated with Alexa Fluor 647 for Spike RBD B1.1, 529 (AFR11056, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, R&D Systems) and conjugated with Alexa Fluor 488 for Full-length spike B1.1, 529 protein (AFG11061, Ala67Val, His69del, Val70del, Thr95Ile, Gly142Asp, Val143del, Tyr144del, Tyr145del, Asn211del, Leu212Ile, ins214Glu-Pro-Glu, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, Thr547Lys, Asp614Gly, His655Tyr, Asn679Lys, Pro681His, Asn764Lys, Asp796Tyr, Asn856Lys, Gln954His, Asn969Lys, Leu981Phe) (Arg682Ser, Arg685Ser, Lys986Pro, Val987Pro, R&D Systems). ..
Article Title: Deficient SARS-CoV-2 hybrid immunity during inflammatory bowel disease.
Article Snippet: .. Detection of SARS-CoV-2 specific memory B cells Spike-specific B cells were detected using either sequential staining of biotinylated Recombinant WT SARS-CoV-2 Spike-Trimer and RBD (HEK) (Miltenyi) conjugated with streptavidin-PE or streptavidin-BV786 respectively were combined with probes already conjugated with Alexa Fluor 647 for Spike RBD B1.1, 529 (AFR11056, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, R&D Systems) and conjugated with Alexa Fluor 488 for Full-length spike B1.1, 529 protein (AFG11061, Ala67Val, His69del, Val70del, Thr95Ile, Gly142Asp, Val143del, Tyr144del, Tyr145del, Asn211del, Leu212Ile, ins214Glu-Pro-Glu, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, Thr547Lys, Asp614Gly, His655Tyr, Asn679Lys, Pro681His, Asn764Lys, Asp796Tyr, Asn856Lys, Gln954His, Asn969Lys, Leu981Phe) (Arg682Ser, Arg685Ser, Lys986Pro, Val987Pro, R&D Systems,). ..
Article Title: Vaccine responses and hybrid immunity in people living with HIV after SARS-CoV-2 breakthrough infections
Article Snippet: .. Spike-specific B cells from thawed PBMCs were detected using sequential staining of biotinylated Recombinant WT SARS-CoV-2 Spike-Trimer and RBD (HEK) (Miltenyi) conjugated with streptavidin-PE or streptavidin-BV786 respectively and combined with probes already conjugated with Alexa Fluor 647 for Spike RBD B1.1, 529 (AFR11056, R&D Systems) and conjugated with Alexa Fluor 488 for Full-length Spike B1.1, 529 protein (AFG11061, R&D Systems,). .. Commercial probes were already conjugated with Alexa Fluor 647 for Spike RBD B1.1, 529 (AFR11056, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, R&D Systems) and conjugated with Alexa Fluor 488 for Full-length Spike B1.1, 529 protein (AFG11061, Ala67Val, His69del, Val70del, Thr95Ile, Gly142Asp, Val143del, Tyr144del, Tyr145del, Asn211del, Leu212Ile, ins214Glu-Pro-Glu, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, Thr547Lys, Asp614Gly, His655Tyr, Asn679Lys, Pro681His, Asn764Lys, Asp796Tyr, Asn856Lys, Gln954His, Asn969Lys, Leu981Phe) (Arg682Ser, Arg685Ser, Lys986Pro, Val987Pro, R&D Systems,).
Article Title: Hybrid and SARS-CoV-2-vaccine immunity in kidney transplant recipients
Article Snippet: .. Spike-specific B cells were detected using either sequential staining of biotinylated Recombinant WT SARS-CoV-2 Spike-Trimer and RBD (HEK) (Miltenyi) conjugated with streptavidin-PE or streptavidin-BV786 respectively were combined with probes already conjugated with Alexa Fluor 647 for Spike RBD B1.1, 529 (AFR11056, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, R&D Systems) and conjugated with Alexa Fluor 488 for Full-length spike B1.1, 529 protein (AFG11061, Ala67Val, His69del, Val70del, Thr95Ile, Gly142Asp, Val143del, Tyr144del, Tyr145del, Asn211del, Leu212Ile, ins214Glu-Pro-Glu, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, Thr547Lys, Asp614Gly, His655Tyr, Asn679Lys, Pro681His, Asn764Lys, Asp796Tyr, Asn856Lys, Gln954His, Asn969Lys, Leu981Phe) (Arg682Ser, Arg685Ser, Lys986Pro, Val987Pro, R&D Systems). ..
Recombinant:Article Title: Hybrid and SARS-CoV-2-vaccine immunity in kidney transplant recipients.
Article Snippet: .. Detection of SARS-CoV-2 specific memory B cells Spike-specific B cells were detected using either sequential staining of biotinylated Recombinant WT SARS-CoV-2 Spike-Trimer and RBD (HEK) (Miltenyi) conjugated with streptavidin-PE or streptavidin-BV786 respectively were combined with probes already conjugated with Alexa Fluor 647 for Spike RBD B1.1, 529 (AFR11056, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, R&D Systems) and conjugated with Alexa Fluor 488 for Full-length spike B1.1, 529 protein (AFG11061, Ala67Val, His69del, Val70del, Thr95Ile, Gly142Asp, Val143del, Tyr144del, Tyr145del, Asn211del, Leu212Ile, ins214Glu-Pro-Glu, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, Thr547Lys, Asp614Gly, His655Tyr, Asn679Lys, Pro681His, Asn764Lys, Asp796Tyr, Asn856Lys, Gln954His, Asn969Lys, Leu981Phe) (Arg682Ser, Arg685Ser, Lys986Pro, Val987Pro, R&D Systems). ..
Article Title: Deficient SARS-CoV-2 hybrid immunity during inflammatory bowel disease.
Article Snippet: .. Detection of SARS-CoV-2 specific memory B cells Spike-specific B cells were detected using either sequential staining of biotinylated Recombinant WT SARS-CoV-2 Spike-Trimer and RBD (HEK) (Miltenyi) conjugated with streptavidin-PE or streptavidin-BV786 respectively were combined with probes already conjugated with Alexa Fluor 647 for Spike RBD B1.1, 529 (AFR11056, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, R&D Systems) and conjugated with Alexa Fluor 488 for Full-length spike B1.1, 529 protein (AFG11061, Ala67Val, His69del, Val70del, Thr95Ile, Gly142Asp, Val143del, Tyr144del, Tyr145del, Asn211del, Leu212Ile, ins214Glu-Pro-Glu, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, Thr547Lys, Asp614Gly, His655Tyr, Asn679Lys, Pro681His, Asn764Lys, Asp796Tyr, Asn856Lys, Gln954His, Asn969Lys, Leu981Phe) (Arg682Ser, Arg685Ser, Lys986Pro, Val987Pro, R&D Systems,). ..
Article Title: Vaccine responses and hybrid immunity in people living with HIV after SARS-CoV-2 breakthrough infections
Article Snippet: .. Spike-specific B cells from thawed PBMCs were detected using sequential staining of biotinylated Recombinant WT SARS-CoV-2 Spike-Trimer and RBD (HEK) (Miltenyi) conjugated with streptavidin-PE or streptavidin-BV786 respectively and combined with probes already conjugated with Alexa Fluor 647 for Spike RBD B1.1, 529 (AFR11056, R&D Systems) and conjugated with Alexa Fluor 488 for Full-length Spike B1.1, 529 protein (AFG11061, R&D Systems,). .. Commercial probes were already conjugated with Alexa Fluor 647 for Spike RBD B1.1, 529 (AFR11056, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, R&D Systems) and conjugated with Alexa Fluor 488 for Full-length Spike B1.1, 529 protein (AFG11061, Ala67Val, His69del, Val70del, Thr95Ile, Gly142Asp, Val143del, Tyr144del, Tyr145del, Asn211del, Leu212Ile, ins214Glu-Pro-Glu, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, Thr547Lys, Asp614Gly, His655Tyr, Asn679Lys, Pro681His, Asn764Lys, Asp796Tyr, Asn856Lys, Gln954His, Asn969Lys, Leu981Phe) (Arg682Ser, Arg685Ser, Lys986Pro, Val987Pro, R&D Systems,).
Article Title: Hybrid and SARS-CoV-2-vaccine immunity in kidney transplant recipients
Article Snippet: .. Spike-specific B cells were detected using either sequential staining of biotinylated Recombinant WT SARS-CoV-2 Spike-Trimer and RBD (HEK) (Miltenyi) conjugated with streptavidin-PE or streptavidin-BV786 respectively were combined with probes already conjugated with Alexa Fluor 647 for Spike RBD B1.1, 529 (AFR11056, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, R&D Systems) and conjugated with Alexa Fluor 488 for Full-length spike B1.1, 529 protein (AFG11061, Ala67Val, His69del, Val70del, Thr95Ile, Gly142Asp, Val143del, Tyr144del, Tyr145del, Asn211del, Leu212Ile, ins214Glu-Pro-Glu, Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His, Thr547Lys, Asp614Gly, His655Tyr, Asn679Lys, Pro681His, Asn764Lys, Asp796Tyr, Asn856Lys, Gln954His, Asn969Lys, Leu981Phe) (Arg682Ser, Arg685Ser, Lys986Pro, Val987Pro, R&D Systems). ..
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